What muscle should I biopsy?

How do I handle the muscle sample?

  • Beginning July 1, 2022, we will only accept formalin fixed samples. We have perfected IHC stains for formalin-fixed tissue and can diagnose equine muscle diseases using these stains on a smaller sample
  • Obtain a 1/2-inch cube of semimembranosus or sacrocaudalis (if atrophy)
  • Leave biopsy in air while you suture
  • Optional, cut the biopsy in half across with width of the muscle (not lengthwise) and place in the cassette so the two halves can be sectioned simultaneously.
  • Place in at least 130 mls (4.5 ounces) 10% neutral buffered formalin 3-5 min after you take the biopsy

When should we schedule a biopsy?

Biopsies can now be scheduled anytime. IHC stains work best on samples that have not been in formalin for more than 5 days.